Supplementary Figure 9.
The DNA damage response pathway is impaired in the SIOD-associated mutant R820H.
(A). Comparison of the CD spectra of wild type ADAAD in the absence and presence of ligands. (B). Comparison of the CD spectra of R595H in the absence and presence of ligands. (C). Interaction of R595H with DNA in the absence and presence of saturating concentration of ATP. (D). Interaction of R595H with ATP in the absence and presence of saturating concentration of DNA. All the titrations were done using fluorescence spectroscopy and represent the average ± standard deviation of two independent experiments. The protein concentration used in these experiments was 0.5 μM. All the data were fitted using one-site saturation model. (E) Formation of 53BP1 was monitored in HeLa cells after treatment with 2 μM doxorubicin for 10 min in the absence and presence of RNase. This data has been reported in [2] and is presented here to show the alteration in number of 53BP1 positive cells when HeLa cells are treated with doxorubicin and RNase.