Table 1. Calculation of kinetic parameters.

Protein	KM (nM)	Vmax (µmol NADH oxidized/min)	kcat (min−1)	kcat/KM (nM−1 min−1)
ADAAD	1.8 ± 0.04	0.5 ± 0.0004	21.5 ± 0.02	11.7 ± 0.3
F507W	6.8 ± 1.8	0.6 ± 0.1	21.8 ± 4.0	3.0 ± 0.2
S558A	6.0 ± 0.4	0.7 ± 0.02	28.9 ± 0.7	4.8 ± 0.3
T560A	7.0 ± 0.6	0.8 ± 0.01	30.4 ± 0.3	4.3 ± 0.3
H594A	36.1 ± 4.5	0.5 ± 0.2	21.5 ± 8.5	0.59 ± 0.2

Data are presented as an average ± s.d. of two independent experiments with each experiment done in duplicates. In all these experiments, 0.1 μM of protein was used.