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. 2018 Jun 27;38(3):BSR20180568. doi: 10.1042/BSR20180568

Table 1. Calculation of kinetic parameters.

Protein KM (nM) Vmax (µmol NADH oxidized/min) kcat (min−1) kcat/KM (nM−1 min−1)
ADAAD 1.8 ± 0.04 0.5 ± 0.0004 21.5 ± 0.02 11.7 ± 0.3
F507W 6.8 ± 1.8 0.6 ± 0.1 21.8 ± 4.0 3.0 ± 0.2
S558A 6.0 ± 0.4 0.7 ± 0.02 28.9 ± 0.7 4.8 ± 0.3
T560A 7.0 ± 0.6 0.8 ± 0.01 30.4 ± 0.3 4.3 ± 0.3
H594A 36.1 ± 4.5 0.5 ± 0.2 21.5 ± 8.5 0.59 ± 0.2

Data are presented as an average ± s.d. of two independent experiments with each experiment done in duplicates. In all these experiments, 0.1 μM of protein was used.