Table 1. Calculation of kinetic parameters.
Protein | KM (nM) | Vmax (µmol NADH oxidized/min) | kcat (min−1) | kcat/KM (nM−1 min−1) |
---|---|---|---|---|
ADAAD | 1.8 ± 0.04 | 0.5 ± 0.0004 | 21.5 ± 0.02 | 11.7 ± 0.3 |
F507W | 6.8 ± 1.8 | 0.6 ± 0.1 | 21.8 ± 4.0 | 3.0 ± 0.2 |
S558A | 6.0 ± 0.4 | 0.7 ± 0.02 | 28.9 ± 0.7 | 4.8 ± 0.3 |
T560A | 7.0 ± 0.6 | 0.8 ± 0.01 | 30.4 ± 0.3 | 4.3 ± 0.3 |
H594A | 36.1 ± 4.5 | 0.5 ± 0.2 | 21.5 ± 8.5 | 0.59 ± 0.2 |
Data are presented as an average ± s.d. of two independent experiments with each experiment done in duplicates. In all these experiments, 0.1 μM of protein was used.