Table 3. K_d values for ATP binding with mutant proteins were calculated using one-site saturation model from the binding data.

	Kd (M)	R2
	Interaction with ATP in the absence of DNA
ADAAD*	(1.6 ± 0.5) × 10−6	0.98
F507A	(1.6 ± 0.4) × 10−6	0.98
F507W	(1.0 ± 0.3) × 10−6	0.98
S558A	(1.9 ± 0.4) × 10-6	0.95
T560A	(1.0 ± 0.3) × 10−6	0.99
R592A	(1.6 ± 0.6) × 10−6	0.98
H594A	(1.6 ± 0.2) × 10−6	0.98
R595A	(1.2 ± 0.2) × 10−6	0.96
R595K	(1.2 ± 0.2) × 10−6	0.97
D591H	(3.2 ± 0.3) × 10−6	0.97
H329D/D591H	(2.8 ± 0.1) × 10−6	0.98
F507A/R592A	(1.7 ± 0.1) × 10−6	0.99
R595H	(1.3 ± 0.2) × 10−6	0.98
	Interaction with ATP in the presence of DNA
ADAAD*	(0.14 ± 0.03) × 10−6	0.98
F507A	(0.45 ± 0.02) × 10−6	0.99
F507W	(0.22 ± 0.03) × 10−6	0.99
S558A	(0.36 ± 0.005) × 10−6	0.98
T560A	(0.22 ± 0.03) × 10−6	0.99
R592A	(0.24 ± 0.02) × 10−6	0.97
H594A	(0.09 ± 0.01) × 10−6	0.99
R595A	(0.11 ± 0.02) × 10−6	0.97
R595K	(0.11 ± 0.04) × 10−6	0.98
D591H	(0.21 ± 0.06) × 10−6	0.99
H329D/D591H	(0.24 ± 0.02) × 10−6	0.96
F507A/R592A	(0.24 ± 0.04) × 10−6	0.98
R595H	(0.23 ± 0.07) × 10−6	0.95

Data are presented as an average ± s.d. of three binding curves.

^*Reported in [24].