Skip to main content
. 2018 Jun 20;9:647. doi: 10.3389/fphar.2018.00647

FIGURE 2.

FIGURE 2

18β-GA inhibits osteoclasts function in vitro. (A) Actin ring structures of osteoclasts and quantification of the actin ring. (B) Pits formation assay of osteoclasts and quantification of pits area. graphic file with name fphar-09-00647-i001.jpg RAW264.7 cells; graphic file with name fphar-09-00647-i002.jpg RAW264.7 cells induced with M-CSF, RANKL, and PBS; graphic file with name fphar-09-00647-i003.jpg RAW264.7 cells induced with M-CSF, RANKL and treated with 6.9525 μg/ml 18β-GA; graphic file with name fphar-09-00647-i004.jpg RAW264.7 cells induced with M-CSF, RANKL and treated with 13.905 μg/ml 18β-GA; graphic file with name fphar-09-00647-i005.jpg RAW264.7 cells induced with M-CSF, RANKL and treated with 27.81 μg/ml 18β-GA. (C) Western blot and optical density analysis of the expression of Cathepsin K, CTR, MMP-9, and TRAP with β-actin as reference (∗P < 0.05, ∗∗P < 0.01 versus graphic file with name fphar-09-00647-i006.jpg).