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. 2018 Jun 20;9:1060. doi: 10.3389/fmicb.2018.01060

FIGURE 3.

FIGURE 3

Assessment of virulence properties of TTSS-PpHLP. Bean leaves were inoculated with Pseudomonas syringae pv. tomato DC3000Δ28E (approximately 105 cfu mL-1) strains expressing HopQ1 or TTSS-PpHLP. Immediately prior to infiltration, bacteria were mixed in a 1:1 ratio. Two and 6 days post-inoculation (dpi), two leaf disks per plant were cut out from the infiltrated zones, ground in sterile 10 mm MgCl2, diluted, and plated on LB medium. Bacterial strains were distinguished by a selectable marker. The CI (competitive index) was calculated as the ratio of bacteria expressing TTSS-PpHLP to bacteria expressing wild-type HopQ1 isolated from plant leaf and normalized to the input titers of the bacteria. Asterisks indicate that the index is significantly different from 1, as established using Student’s t-test (P < 0.001). Pluses represent the means. The experiment was performed three times with similar results.