Table 2.
Rhizobial isolates from faba bean in Panxi, their genetic and symbiotic characteristics and phylogenetic affiliation.
| Isolatea | Sampling siteb | 16S rDNA RFLP genotypec | IGS RFLP genotypec | CACAI genotype | CACAI groupd | MLSAe | Plant shoot dry mass (g plant−1)f | No. of nodules per plant |
|---|---|---|---|---|---|---|---|---|
| SCAUf129 | 21 | a | 1 | 1 | A | 1.593 ± 0.340↑* | 34.3 | |
| SCAUf141 | 17 | a | 17 | 5 | A | 1.523 ± 0.020↑* | 41.7 | |
| SCAUf142 | 4 | a | 1 | 1 | A | 1.457 ± 0.247↑* | 93.0 | |
| SCAUf110 | 11 | a | 21 | 9 | B | 1.397 ± 0.301↑* | 18.7 | |
| SCAUf126 | 12 | a | 1 | 1 | A | 1.377 ± 0.074↑* | 25.0 | |
| SCAUf118 | 7 | a | 1 | 1 | A | 1.375 ± 0.025↑* | 62.0 | |
| SCAUf114 | 12 | a | 1 | 1 | A | 1.327 ± 0.186↑* | 40.3 | |
| SCAUf122 | 14 | a | 1 | 1 | A | 1.287 ± 0.156↑* | 39.7 | |
| SCAUf123 | 9 | g | 22 | 24 | C | 1.283 ± 0.063↑* | 43.3 | |
| SCAUf113 | 22 | a | 1 | 1 | A | 1.280 ± 0.265↑* | 94.3 | |
| SCAUf148 | 16 | h | 20 | 25 | D | 1.267 ± 0.201↑* | 52.0 | |
| SCAUf131 | 25 | a | 1 | 1 | A | R. anhuiense CCBAU 23252T (98.5%) | 1.215 ± 0.365 | 98.5 |
| SCAUf125 | 18 | a | 23 | 10 | E | 1.135 ± 0.095 | 61.5 | |
| SCAUf136 | 9 | a | 1 | 1 | A | 1.130 ± 0.160 | 58.7 | |
| SCAUf111 | 21 | a | 1 | 1 | A | 1.127 ± 0.109 | 72.3 | |
| SCAUf133 | 11 | a | 19 | 7 | B | R. sophorae CCBAU 03386T (96.6 %) | 1.120 ± 0.210 | 17.0 |
| SCAUf102 | 2 | a | 1 | 1 | A | 1.097 ± 0.138 | 28.3 | |
| SCAUf106 | 1 | a | 5 | 13 | F | R. vallis CCBAU 65647T (93.0%) | 1.095 ± 0.088 | 48.0 |
| SCAUf109 | 1 | a | 4 | 12 | G | R. vallis CCBAU 65647T (93.0%) | 1.094 ± 0.087 | 46.7 |
| SCAUf139 | 4 | a | 1 | 1 | A | 1.087 ± 0.121 | 40.3 | |
| SCAUf150 | 16 | h | 20 | 25 | D | A. radiobacter NCPPB 2437T (97.3%) | 1.080 ± 0.131 | 4.0 |
| SCAUf121 | 23 | a | 18 | 6 | A | 1.050 ± 0.044 | 44.7 | |
| SCAUf134 | 15 | a | 19 | 7 | B | 1.017 ± 0.153 | 27.0 | |
| SCAUf115 | 7 | a | 1 | 1 | A | 0.950 ± 0.410 | 22.5 | |
| SCAUf87 | 19 | c | 8 | 19 | H | A. radiobacter NCPPB 2437T (97.3%) | 0.934 ± 0.146 | 23.0 |
| SCAUf137 | 23 | a | 18 | 6 | A | 0.933 ± 0.206 | 35.0 | |
| SCAUf101 | 2 | a | 1 | 1 | A | 0.930 ± 0.021 | 27.7 | |
| SCAUf143 | 15 | a | 19 | 7 | B | 0.923 ± 0.228 | 22.5 | |
| SCAUf147 | 15 | a | 19 | 7 | B | 0.893 ± 0.173 | 22.0 | |
| SCAUf146 | 12 | a | 1 | 1 | A | 0.870 ± 0.150 | 4.5 | |
| SCAUf99 | 20 | a | 6 | 14 | E | R. sophorae CCBAU 03386T (96.6 %) | 0.864 ± 0.041 | 59.7 |
| SCAUf92 | 1 | a | 2 | 8 | A | 0.856 ± 0.099 | 23.7 | |
| SCAUf132 | 21 | a | 1 | 1 | A | 0.833 ± 0.231 | 16.7 | |
| SCAUf103 | 19 | f | 15 | 22 | I | 0.820 ± 0.066 | 68.0 | |
| SCAUf107 | 13 | a | 12 | 4 | J | 0.815 ± 0.066 | 3.0 | |
| SCAUf149 | 11 | i | 25 | 26 | K | A. radiobacter NCPPB 2437T (97.3%) | 0.805 ± 0.155 | 35.0 |
| CK | – | – | – | 0.801 ± 0.139 | 0.0 | |||
| SCAUf95 | 5 | a | 1 | 1 | A | 0.790 ± 0.169 | 20.0 | |
| SCAUf120 | 17 | a | 17 | 5 | A | 0.790 ± 0.030 | 25.0 | |
| SCAUf90 | 10 | a | 11 | 3 | B | R. sophorae CCBAU 03386T (96.6 %) | 0.785 ± 0.051 | 60.3 |
| SCAUf94 | 8 | a | 9 | 16 | L | R. vallis CCBAU 65647T (93.0%) | 0.780 ± 0.060 | 18.7 |
| SCAUf86 | 13 | b | 12 | 17 | J | R. sophorae CCBAU 03386T (99.3 %) | 0.770 ± 0.121 | 12.7 |
| SCAUf124 | 14 | a | 1 | 1 | A | 0.753 ± 0.067 | 36.0 | |
| SCAUf138 | 14 | a | 1 | 1 | A | 0.750 ± 0.057 | 38.0 | |
| SCAUf145 | 18 | a | 17 | 5 | A | 0.750 ± 0.110 | 35.5 | |
| SCAUf140 | 17 | a | 18 | 6 | A | R. anhuiense CCBAU 23252T (98.9%) | 0.743 ± 0.170 | 23.0 |
| SCAUf117 | 22 | a | 1 | 1 | A | 0.737 ± 0.177 | 71.7 | |
| SCAUf127 | 22 | a | 1 | 1 | A | R. anhuiense CCBAU 23252T (98.9%) | 0.733 ± 0.065 | 41.3 |
| SCAUf128 | 9 | a | 1 | 1 | A | 0.707 ± 0.188 | 13.3 | |
| SCAUf100 | 10 | e | 13 | 21 | M | R. vallis CCBAU 65647T (99.0%) | 0.689 ± 0.034 | 11.0 |
| SCAUf104 | 24 | f | 16 | 23 | I | R. anhuiense CCBAU 23252T (99.2%) | 0.680 ± 0.082 | 15.0 |
| SCAUf89 | 20 | a | 10 | 2 | B | 0.680 ± 0.060 | 21.3 | |
| SCAUf119 | 4 | a | 1 | 1 | A | 0.667 ± 0.188 | 20.0 | |
| SCAUf135 | 4 | a | 1 | 1 | A | 0.663 ± 0.114 | 16.7 | |
| SCAUf105 | 3 | a | 3 | 11 | A | R. anhuiense CCBAU 23252T (99.3%) | 0.660 ± 0.020 | 5.0 |
| SCAUf108 | 3 | a | 7 | 15 | A | 0.650 ± 0.031 | 6.3 | |
| SCAUf116 | 25 | a | 1 | 1 | A | 0.647 ± 0.189 | 47.0 | |
| SCAUf112 | 21 | a | 1 | 1 | A | 0.647 ± 0.099 | 11.7 | |
| SCAUf96 | 5 | a | 1 | 1 | A | 0.643 ± 0.070 | 21.3 | |
| SCAUf88 | 19 | a | 2 | 8 | A | 0.632 ± 0.073 | 15.0 | |
| SCAUf144 | 4 | b | 24 | 18 | C | R. fabae CCBAU 33202T (99.9 %) | 0.605 ± 0.090 | 24.0 |
| SCAUf97 | 19 | a | 1 | 1 | A | 0.573 ± 0.032 | 31.0 | |
| SCAUf91 | 6 | a | 2 | 8 | A | R. anhuiense CCBAU 23252T (99.8%) | 0.533 ± 0.087 | 13.0 |
| SCAUf130 | 23 | a | 18 | 6 | A | 0.532± 0.086 | 23.0 | |
| SCAUf93 | 19 | d | 14 | 20 | N | A. radiobacter NCPPB 2437T (97.3%) | 0.532± 0.069 | 0 |
| SCAUf98 | 19 | a | 1 | 1 | A | 0.517± 0.059 | 0 |
CK: uninoculated treatment in the symbiotic efficiency test. Representative isolates for sequencing in bold.
Sampling sites are the same as Figure 1.
Genotype: the combination of the restriction patterns obtained by enzymes MspI, HaeIII, TaqI, and HinfI.
CACAI: clustered analysis of combined ARDRA and IGS-RFLP, Groups were defined at 94.5% similarity level.
MLSA, multilocus sequence analysis of combined recA, atpD, and glnII. The percentages are sequence similarities to the closely related species or the closest type strain.
Type strain.
Significantly higher shoot dry mass than that in CK treatment according to the LSD test (P = 0.05). Data presented as mean value ± standard deviation (n = 3, except n = 2 for SCAUf93 and SCAUf98).