FIGURE 4.

Improved learning and memory in 5xFAD-Thy1-Cre mice (pt. 1). (A) Experimental set-up of the active place avoidance test. Mice are placed on a rotating platform and visual cues outline the R0 shock area. Upon entering the R0 area, mice receive mild electric stimuli until leaving the area. An initial trial (habituation) without electric stimulation is followed by eight training trials with electric stimulation. One recall trial without shocks is carried out 24 h later. (B–E) Mice were tracked during each trial and the following parameters were monitored: visits/distance (B), latency to first R0 entry (C), time spent in R0 (D), and number of shocks received (E). Learning to avoid R0 during the training trials is improved in 5xFAD-Thy1-Cre mice, compared to 5xFAD mice (n = 8 control; 7 5xFAD; 10 Thy1-Cre; 9 5xFAD-Thy1-Cre mice). Additionally, the respective area under the curve (AUC) was determined for each mouse, and resulting means of the four mouse groups are depicted. Means ± SEM. Statistical analysis for all groups and for each time point was performed by one-way ANOVA with Tukey’s test for multiple comparison (confidence interval = 95%). Consequently, in (B–E), the red stars denote the significance for the comparison 5xFAD vs. 5xFAD-Thy1-Cre mice.