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. 2018 Jun 20;12:415. doi: 10.3389/fnins.2018.00415

FIGURE 1.

FIGURE 1

Tau level-dependent neuronal dysfunction in WT4R-tau-Tg worms. Mock-Tg (a), WT4R(L)-Tg (b), WT4R(H)-Tg (c), WT4R(ExH1)-Tg (d), and WT4R(ExH2)-Tg (e) worms were grown on NGM plates and subjected to (A) Western blotting of tau (pool-2) and tubulin (DM1A) in the total lysate. Expression levels were quantified and shown against WT4R(L)-Tg (Lower panel). Average of four independent experiments are shown (means ± SEM, n = 4). Statistical significance was analyzed by Tukey’s post hoc test (∗∗p < 0.01, ∗∗∗p < 0.001, vs. WT4R(L)-Tg). (B) The behavioral analysis of uncoordinated movement in each worm line was analyzed. Upper panel shows the population of healthy (open), weak Unc (hatched), and severe Unc (closed) worms (n = 60) in each line. Lower panel shows that the severity of Unc as described in Materials and Methods section. Average scores of three independent experiments are shown (means ± SEM, n = 3). Statistical significance was analyzed by Tukey’s post hoc test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, vs. Mock-Tg).