Skip to main content
. 2018 Jun 20;12:415. doi: 10.3389/fnins.2018.00415

FIGURE 2.

FIGURE 2

Tau expression enhances uncoordinated movement induced by tbce-1 gene knock down. (A) WT4R(L)-Tg/rrf-3 (a–c) and Mock-Tg/rrf-3 worms (d) were grown on L4440::T (a; control) or L4440:: tbce-1 (b–d; tbce-1) RNAi plates from embryo to 3 days after hatch. Rrf-3 background worms grown on tbce-1 RNAi plates showed Unc (b) or a frequent burst phenotype (c). (B) The severity of Unc was assessed as described in Materials and Methods section. Average scores of three independent experiments are shown (means ± SEM, n = 3). WT4R(L)-Tg/rrf-3 worms showed a significantly higher sensitivity to tubulin chaperon knockdown. (C) The levels of tbce-1 mRNA were quantified by quantitative real time RT-PCR (means ± SEM, n = 3). tbce-1 mRNA is suppressed to less than 25% compared to controls, regardless of tau expression. (D) The levels of tau and α-tubulin were analyzed by Western blotting. The percent expression of control worms is shown (means ± SEM, n = 3). Statistical significance was analyzed by Tukey’s post hoc test (∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001) or Student’s t-test (∗p < 0.05).