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. 2018 Jun 20;12:415. doi: 10.3389/fnins.2018.00415

FIGURE 3.

FIGURE 3

Tau expression in mechanosensory neurons exacerbates touch sense abnormality induced by tubulin knockdown. (A) WT4R(L)-Tg/rrf-3 (a–c) and Mock-Tg/rrf-3 (d–f) worms were subjected to immunolabeling using 6-11B1 (anti-mec-12; a and d), and pool-2 (anti-tau; b and e). Merged views are also shown (c and f). Human tau was expressed in as pan-neuronal pattern (b and e) including PLMs (arrows in a–c) in lumber ganglia and ALMs (arrowheads in d–f) in the abdomen. Scale bars = 30 μm. (B) Mock-Tg/rrf-3 (open) and WT4R(L)-Tg/rrf-3 (solid) worms were grown on L4440::T (control) or L4440::mec-12 RNAi plates from embryo to 4 days after hatch. Touch sensitivity was analyzed as described in the Materials and Methods section (means ± SEM, n = 60). (C) The expression of mec-12 mRNA in the indicated worms was quantified by quantitative real time RT-PCR (means ± SEM, n = 3). Statistical significance was analyzed by Tukey’s post hoc test (∗p < 0.05, ∗∗∗p < 0.001).