FIGURE 6.

In vitro dephosphorylation restores the MT-binding activity of human-tau in tau-Tg worms. (A) l-phosphatase dephosphorylation of tau in worms was assessed by Western blotting using the indicated antibodies. (B) Purified tau from WT4R(H)-Tg worms were remixed with recombinant human 0N4R tau isoform (h-tau, positive control) and MTs from naive worms (total). MT-unbound and MT-bound fractions were prepared from this mixture as described in the Materials and Methods section. Bands corresponding to phosphorylated tau (P-tau, open arrowheads), dephosphorylated tau (DeP-tau, closed arrowheads), and recombinant tau (h-tau, arrows) are indicated. Note that a portion of dephosphorylated tau, but not phosphorylated tau, was recovered in the MT-bound fraction like recombinant tau.