Table 1.

Features of the generated metagenomic libraries.

Metagenomic library	USP 1	USP 3
Total number of clones	100,000	90,000
Percentage of clones with insert (%)	60	70
Number of clones with insert	60,000	63,000
Total number and rate* of fluorescent clones	400 (1:150)	700 (1:90)
Total number and rate* of green clones	270 (1:220)	400 (1:157)
Total number and rate* of red clones	130 (1:460)	300 (1:210)
Average insert size (kb)	4.5	3.7
Total metagenomic library size (Mb)	270	233
Estimated number of genomes**	60	52

^*Rate represented by the number of fluorescent clones divided by the total number of clones with inserts.

^**Assuming 4.5 Mb per genome (Raes et al., 2007).