Table 2.
Description of the ORFs contained in plasmids from the selected clones (pCAW1 to pCAW10) and their sequence similarities.
| Clone_Sample [insert bp] | G + C % | GenBank accession No. | Phyluma | ORFb | Strand | Length (aac) | Closest similar proteind (Length in aa) | Closest Organism/Phylume | Identity (%) | Putative function |
|---|---|---|---|---|---|---|---|---|---|---|
| pCAW1 (2,367 bp) | 55% | KY939589 | Proteobacteria or Verrucomicrobia | 1 | Minus | 131 | Hypothetical protein (416) | Bacteriodetes bacterium/Proteobacteria | 68% | Alginate lyase |
| 2 | Plus | 271 | Hypothetical protein (261) | Acidobacteria bacterium/Acidobacteria | 73% | 17-B-hydroxysteroid dehydrogenase | ||||
| 3b | Plus | 295 | Beta-glucosidase (777) | Caulobacter sp. OV484/Proteobacteria | 66% | Beta-glucosidase | ||||
| pCAW2 (2,069 bp) | 52% | KY939590 | Actinobacteria | 1 | Plus | 304 | Unkonwnc | Hyphomicrobium sp. NDB2Meth4/Proteobacteria | 33% | Unknown |
| 2 | Plus | 249 | Unkonwn | Hungatella hathewayi/Firmicutes | 33% | Unknown | ||||
| pCAW3 (4,404 bp) | 53% | KY939591 | Proteobacteria | 1 | Minus | 318 | IS4 family Transposase (320) | Escherichia coli/Proteobacteria | 96% | IS4 family transposase |
| 2 | Minus | 1011 | DNA-directed RNA polymerase subunit beta' (1430) | Sphingobacteriales bacterium 44-61/Bacteroidetes | 83% | RNA polymerase - Beta Subunit | ||||
| 3 | Plus | 120 | Uncharacterised protein (135) | Bordetella pertussis/Proteobacteria | 47% | Unknown | ||||
| 4 | Plus | 151 | Uncharacterised protein (130) | Bordetella pertussis/Proteobacteria | 37% | Unknown | ||||
| 5 | Plus | 94 | Uncharacterised protein (64) | Bordetella pertussis/Proteobacteria | 82% | Unknown | ||||
| 6 | Plus | 96 | Uncharacterised protein (86) | Vibrio cholerae/Proteobacteria | 48% | Unknown | ||||
| 7 | Plus | 173 | predicted protein (585) | Ruminococcus sp. CAG:403/Proteobacteria | 26% | Unknown | ||||
| pCAW4 (4,002 bp) | 61% | KY939592 | Proteobacteria | 1 | Minus | 245 | Nosine monophosphate cyclohydrolase (246) | Ktedonobacter racemifer/Chloroflexi | 63% | IMP cyclohydrolase |
| 2 | Minus | 214 | Phosphodiesterase (498) | Candidate division NC10 bacterium/NC10 | 40% | Phosphodiesterase | ||||
| 3 | Minus | 402 | Hypothetical protein A2Y08_02680 (625) | Planctomycetes bacterium GWA2_40_7/Planctomycetes | 43% | Unknown | ||||
| 4b | Plus | 142 | Gentisate 1,2-dioxygenase (349) | Pseudomonas sp. 21C1/Proteobacteria | 60% | Gentisate 1,2-dioxygenase | ||||
| pCAW5 (2,724 bp) | 54% | KY939593 | Verrucomicrobia | 1b | Plus | 642 | Pyruvate:ferredoxin oxidoreductase (1565) | Uncultured bacterium HF770_11D24]/Acidobacterium | 80% | Pyruvate:ferredoxin oxidoreductase |
| pCAW6 (2,125 bp) | 57% | KY939594 | Chloroflexi or Proteobacteria | 1 | Plus | 159 | Hypothetical protein BGO39_33875 (215) | Chloroflexi bacterium 54-19/Chloroflexi | 65% | MerR family |
| 2 | Plus | 336 | Hypothetical protein BGO39_33870 (347) | Chloroflexi bacterium 54-19/Chloroflexi | 78% | PrsW intramembrane metalloprotease | ||||
| 3b | Plus | 163 | Hypothetical protein BGO39_33865 (173) | Chloroflexi bacterium 54-19/Chloroflexi | 75% | Chromate transporter | ||||
| pCAW7 (2,558 bp) | 46% | KY939595 | Actinobacteria | 1b | Minus | 391 | Hypothetical protein A2X07_06330 (480) | Flavobacteria bacterium GWF1_32_7/Bacteroidetes | 45% | Por secretion system sorting domain |
| 2 | Minus | 250 | Hypothetical protein (586) | Chitinophagaceae bacterium PMP191F/Bacteroidetes | 65% | Polysaccharide Lyase | ||||
| pCAW8 (4,480 bp) | 57% | KY939596 | Actinobacteria | 1 | Plus | 508 | Hypothetical protein AUH20_02325 (597) | Rokubacteria bacterium/Rokubacteria | 76% | 5-oxoprolinase / Hydantoinase_B |
| 2 | Minus | 348 | Oxidoreductase (336) | Rokubacteria bacterium/Rokubacteria | 61% | Flavin-utilizing monoxygenases | ||||
| 3 | Plus | 314 | Hypothetical protein ETSY1_46935 (279) | Candidatus Entotheonella sp. TSY1/Tectomicrobia | 76% | Cellulose biosynthesis BcsQ | ||||
| pCAW9 (2,573 bp) | 43% | KY939597 | Bacteroidetes or Proteobacteria | 1b | Minus | 81 | Hypothetical protein (129) | Janthinobacterium/Proteobacteria | 50% | Unknown |
| 2 | Minus | 303 | Formylglycine-generating enzyme (379) | Mucilaginibacter sp./Bacteroidetes | 65% | Formylglycine-generating enzyme | ||||
| 3 | Minus | 457 | Acetylglucosamine-6-sulfatase (504) | Flavihumibacter solisilvae/Bacteroidetes | 67% | Acetylglucosamine-6-sulfatase | ||||
| pCAW10 (2,076 bp) | 56% | KY939598 | Proteobacteria | 1 | Plus | 204 | Hypothetical protein (195) | Luminiphilus syltensis/Proteobacteria | 50% | Unknown |
a
Classification based on PhylopythiaS (Patil et al., 2012) webserver
b
Truncated proteins
c
aa, amino acids
d
Sequences with an E-value higher than 0.001 in Blastp searches were considered to be unknown proteins
e
Classification based on Blastp.