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. Author manuscript; available in PMC: 2018 Nov 21.
Published in final edited form as: Nat Mater. 2018 May 21;17(7):642–651. doi: 10.1038/s41563-018-0083-8

Figure 3. Post-stroke neurogenesis and axonal sprouting.

Figure 3

(A) Fluorescent images of neuroblasts (Dcx) and the proliferation marker BrdU, and (B) axonal neurofilaments (NF200) in and around the stroke site (*) at 2 weeks and (G) at 16 weeks after gel transplantation. (C) Quantification of neuroblasts (Dcx) and proliferating neuroblasts (Dcx/BrdU) in the ipsilateral ventricle, (D) the number of neuroblasts migrating and their migration distance and number, (E) the axonal area (NF200) in and around the stroke site, and (F) infiltration distance and penetration angle in the stroke site. (H) Quantification of axonal area (NF200) in and (I) around the stroke site, (J) infiltration distance and (K) axonal penetration angle 16 weeks after gel injection. In all plots, the dotted red line and red number indicates the value for the give quantification of the contralateral side. Empty gel = gel = HA hydrogel, Vs = 200 ng of soluble VEGF, lcV = 1μg nH loaded with 200 ng VEGF, hcV = 0.01 μg nH loaded with 200 ng VEGF and 0.99 μg unloaded nH, Endo = a daily i.p injection of endostatin day 5 to 15. Data is presented using a min to max box plot. Each dot in the plots represents one animal and p values were determined by One-way ANOVA with a Tukey’s post-hoc test, with *, ** and **** indicating p < 0.05, p < 0.01 and p < 0.0001, respectively. Scale bar: 100 μm.