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. Author manuscript; available in PMC: 2019 Jan 2.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Jun 5;57(27):8149–8153. doi: 10.1002/anie.201803907

Figure 2.

Figure 2

High-resolution mass spectra of WT (A–C) and F2-Tyr222 ADO (D–F). In WT ADO, both the crosslinked form at m/z 427.6680 and the uncrosslinked form at m/z 457.1865 were observed by LC-MS/MS (A). The predicted values are 427.6661 and 457.1847. CID spectra of the uncrosslinked (B) and crosslinked (C) forms identified the crosslink between Cys220 and Tyr222. In F2-Tyr222 ADO, both the crosslinked form at m/z 436.6638 and the uncrosslinked form at m/z 475.1778 were observed by LC-MS/MS (D). The predicted values are 436.6614 and 475.1753. CID spectra of uncrosslinked (E) and crosslinked (F) forms identified the crosslink was formed by Cys220 and F2-Tyr222 with only one fluorine. C* represents the carbamido-methylated cysteine. Arc represents crosslinking. The intact peptides (z = +2) are highlighted by red; specified fragments are shown in black while other observed values are in gray.