A. Quantification of tumor/lesions by histology. Each of the lobes of the lung were examined and tumors quantified (left). Representative lung slices stained with hematoxylin and eosin (5x and 20x magnification) from a representative adjuvant treated and a vaccinated mouse. Data is pooled between two independent runs of the experimental protocol.
B. Quantification of tumor burden by MRI in adjuvant versus vaccinated animals. Average lung intensity is defined as mean pixel intensity x area/total lung area quantified (left). Representative images (2 slices) from an adjuvant treated and vaccinated animal (middle, right) obtained at 10 weeks post-EGFRL858R transgene induction. Data represents a subset of animals selected for imaging from the second run of the bioassay.