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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Arthritis Rheumatol. 2018 May 27;70(7):1030–1041. doi: 10.1002/art.40456

Figure 1. Increased ubiquitin and polyubiquitinated substrate levels in human knee OA chondrocytes.

Figure 1

Equal aliquots (10 µg) of lysates from normal (Grade 0/Grade I) and Grade IV OA human articular chondrocytes were used for 4–12% SDS-PAGE/Western blot analysis, employing antibodies directed against ubiquitin (Ub, n=16) (A) and K48-linked ubiquitin (n=18) (B). Equal protein loading was also confirmed by blotting for β-actin.