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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Arthritis Rheumatol. 2018 May 27;70(7):1030–1041. doi: 10.1002/art.40456

Figure 5. PSMD11 siRNA knockdown induces increased NO release, induces decreases in SOX9 and AGC1, and stimulates elements of the autophagy process, in normal chondrocytes.

Figure 5

We studied PSMD11 siRNA knockdown effects on NO and MMP13 release in response to IL-1β (10 ng/ml) in normal human knee chondrocytes (A). We also analyzed PSMD11 siRNA effects on SOX9 and AGC1 (B), and SOX9 protein expression, as well as autophagy-related LC3A/B-I to LC3A/B-II conversion, the autophagy-UPS adaptor and LC3-binding protein p62 (C), and perinuclear co-localization of p62 and LC3A/B-I to LC3A/B-II in normal chondrocytes (D), with 300 cells/treatment counted to quantify structures where LC3A/B and p62 co-localized. Analysis was by 2-way ANOVA (Multiple comparison with Bonferroni post hoc comparison).