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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Arthritis Rheumatol. 2018 May 27;70(7):1030–1041. doi: 10.1002/art.40456

Figure 6. Effects of selective PSMD11 gain of function in cultured human knee OA chondrocytes.

Figure 6

(A) Transfecting Grade IV OA chondrocytes, we assessed effects of HA tag-PSMD11 plasmid, compared to control plasmid transfection, on proteasome activity (n=4 donors), and (B) on accumulation of K48-polyubiquitinated proteins (results representative of 4 donors). (C) PSMD11 gain of function by transfection in OA chondrocytes (n=5 different donors), validated to augment cellular HA, was assessed for effects on SOX9 levels. (D) Using ChIP assay, we separately analyzed the effect of PSMD11 gain of function on nuclear SOX9 binding to the AGC1 promoter and COL2A1 enhancer.