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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Shock. 2018 Dec;50(6):671–676. doi: 10.1097/SHK.0000000000001089

Figure 3. Thrombin provokes the release of active PAI-1, capable of complexing with t-PA.

Figure 3

Supernatant and lysate were combined with t-PA (150ng/ml) followed by measurement of t-PA-PAI-1 complex by ELISA. Thrombin-treated supernatant demonstrate a 3-4 fold increase in complex formation compared to control demonstrating release of active PAI-1. Conversely, control lysate had a 5-6-fold increase in complex formation compared to thrombin-treated, confirmed storage of active PAI-1. n=3-4, values reported as median. *p<0.03 compared to control lysate (same time point). #p<0.002 compared to thrombin supernatant (same time point).