Table 1.

t-PA-challenge TEG

	R-time (min)	Angle (degrees)	MA (mm)	LY30 %
Whole blood	11.2 (8.1-13.0)	39.3 (35.3-47.4)	35.4 (27.2-57.5)	15.9 (11.7-19.3)
Normal saline	9.6 (7.5-10.5)	51.3 (42.4-58.8)	38 (25.6-52.4)	35.5 (27.0-50.7)
Control lysate- 15m	6.1 (5.4-7.8)	55.5 (46.6-60.4)#	48.9 (35.4-57.8)§	7.9 (4.3-11.8)*
1IU Thrombin lysate- 15m	5.9 (3.7-6.9)†	57.0 (47.4-63.6)	59.0 (38.6-65.0)	12.4 (11-21.1)
5IU Thrombin lysate- 15m	1.7 (1.4-2.7)†	59.3 (51.8-64.3)	42.2 (22.9-61.1)	28.5 (17.0-34.1)
Control lysate- 30m	6.4 (5.5-7.6)	51.3 (42.9-53.8)#	47.6 (37.7-58.8)§	6.1 (5.1-12.3)*
1IU Thrombin lysate- 30m	2.0 (1.8-4.4)†	53.4 (42.9-61.1)	58.0 (51.3-62.9)	13.7 (8.7-16.9)
5IU Thrombin lysate- 30m	2.0 (1.8-4.4)†	60.7 (48.2-64.1)	40.9 (23.0-57.9)	29.6 (19.2-41.1)

Thrombelastography variables for whole blood, normal saline dilution (20% dilution of whole blood), control and thrombin (1IU, 5IU/ml) platelet lysate at 15 minutes, and control and thrombin (1IU, 5IU/ml) platelet lysate at 30 minutes (lysate in normal saline, 20% dilution of whole blood) in the presence of exogenous t-PA (75ng/ml). Thrombin lysate, with internalized thrombin, demonstrated reduced R-time compared to the control platelet lysate. In addition, control platelet lysate had an increased angle and MA compared to whole blood. Control platelet lysate, with preserved PAI-1, inhibited tPA-stimulated TEG fibrinolysis, with a reduced LY30 compared to normal saline and both thrombin platelet lysates (1IU, 5IU/ml) after degranulation of PAI-1. Control platelet lysate also demonstrated reduced LY30 compared to whole blood.

^*p<0.01 compared to normal saline, whole blood, and thrombin lysates (5IU, 1IU) LY30 at the same time point.

^†p<0.01 compared to control platelet lysates R-time at the same time point.

^#p<0.002 compared to whole blood angle.

^§p<0.02 compared to whole blood angle.