| 1 M Sucrose | 45.0 μL |
| 10x GpC Buffer | 5.0μL |
| Nuclei (250,000) | 94.5μL |
| 32 mM SAM | 1.5μL |
| 50 U/ μl M.CviPI | 4.0μ L |
| ---------------------------- | |
| Total | 150.0 μL/tube |
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