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. 2017 Nov 22;9(7):616–628. doi: 10.1007/s13238-017-0492-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Depletion of Sec61β improves the lateral mobility of translocon complexex. (A) M3/18 cells transfected with siRNA of Sec61β or Climp63 for 48 h were lyzed for analysis after FRAP. The levels of Sec61β were determined by Western blotting, with actin as a loading control. Climp63 and Actin by RT-PCR were resolved by agarose gel. (B) M3/18 cells in (A) were analyzed by fluorescence recovery after photobleaching assay. The initial fluorescence intensity was set at 100%. Data are mean ± SEM. (n =7 for control group, 6 for siSEC61B group and 5 for siClimp63 group). (C) M3/18 cells were transfected with siRNA of Sec61β for 48 h. The lysates were precipitated with anti-GFP antibody. The levels of indicated proteins were analyzed with Western blotting