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Sec61β stabilizes membrane attachment of ribosomes. (A) Polysome profiles of U2OS cells transfected control siRNA (siCtrl) or siSEC61B#2. (B) Total lysates from (A) were analyzed by Western blotting, with actin as a loading control. (C) As in (A), but U2OS cells co-transfected siSEC61B#2 and indicated plasmids. (D) As in (B), but from cells in (C). For all of them, the trends are similar among at least two independent repeats
