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. 2018 May 31;18:33–42. doi: 10.1016/j.redox.2018.05.014

Fig. 1.

Fig. 1

Impact of levobupivacaine and etoposide on cell proliferation. (A) Cell number of BHP and DU145 cell lines after 24 h treatments with 1 mM of levobupivacaine or 50 µM etoposide. (B) Cell number of PC3 cell line after 24 h treatments with 1 mM of levobupivacaine or 50 µM etoposide. (C) Cell death induction by levobupivacaine was assessed by following the expression level of PARP or (D) Caspase activity on cells treated with levobupivacaine (Levo) or untreated (Untr). The effect of 50 µM etoposide on Caspase activity has also been assessed. (E) Expression level of Akt and P-AKT. (F) Cell cycle arrest induced by levobupivacaine was determined in DU145 using flow cytometry analysis (BrDU and 7-AAD staining). (G) Effect of levobupivacaine pre-treatment on cell proliferation rate. Cell enumeration was performed over 48 h of growth after 24 h of treatment and subsequent wash-off. All the data shown correspond to the mean value ± SD of N ≥ 3 different experiments. Significantly different from the untreated cells at: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.