Fig. 5.

Impact of levobupivacaine of autophagy induction. (A) Autophagy induction was assessed by following the activation of LC3, as assessed by the expression ratio between LC3B I and LC3B II, on BHP and DU145 cell lines after 24 h of treatment with 1 mM levobupivacaine. (B) Enumeration of BHP and DU145 cell lines after 24 h of treatment with 1 mM levobupivacaine co-treated or not with 100 nM Wortmannin. Results are expressed as percentage of control. All the data shown correspond to the mean value ± SD of N ≥ 3 different experiments. Significantly different from the untreated cells at: * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.