Table 1.
Primers used for the amplification of the full-length CSFV
| Fragment | Primer sequence (5′-3′) a | Position in genomea | Product size (bp)a |
|---|---|---|---|
| CSFV-A | GTATACGAGGTTAGTTCATTCTCGT GATTACCAGAGAAAGCAACAAGAAT |
1-2047 | 2047 |
| CSFV-B | GATAATAGGCCCCGGTAAATTTGAC TTTCCTTACAGGTCCCTCGCTAGAG |
2023–3313 | 1291 |
| CSFV-C | AAATGAGACGGGTTACAGGGTA CATCCCGTAGATCTCTTCACCTCCA |
3124–4771 | 1648 |
| CSFV-D | CATAGATGAAATAGCTGGCGGGACC TAGTGCTCTGCCAGCCTCCACAGTG |
4564–7171 | 2608 |
| CSFV-E | TCTGCTGATATCAGAGGAGCTG GCTTACCCAGACTTAATGTTTCTAG |
6866–9668 | 2803 |
| CSFV-F | GCCCTATGTAAGGTCGACACCGCTC GGGCCGTTAGGAAATTACCTTAGTC |
9572–12,296 | 2725 |
aThe sequence of primer sequence, position in genome and product size with respect to the CSFV Zj0801 (accession no. FJ529205) genome