Figure 2.

Chromosomes 1 and 2 Are Highly Prone to Lagging After Nocodazole Washout

(A) RPE1 cells were treated with 8 hr nocodazole, then released for 1 hr before FISH with specific centromere enumeration probes as indicated.

(B) Segregation error rates and average number of lagging chromosomes (errors) per erroneous anaphase.

(C) Percentage erroneous RPE1 anaphases (one or more lagging chromosomes) exhibiting lagging of chromosomes indicated.

(D) Quantification of percentage of lagging chromatids that are the chromosome indicated from erroneous anaphases. Total lagging chromatids were scored using DAPI-positive chromatid counting. Expected frequency is calculated using 1/23, assuming a random distribution among the 23 human chromosomes. (C) and (D) show mean ± SD of three independent experiments (except chromosome 17; two experiments), 268–481 lagging chromosomes analyzed per chromosome.

(E) FISH of BJ cells after nocodazole treatment as in (A).

(F) Segregation error rates and average number of errors per erroneous anaphase.

(G) Percentage erroneous BJ anaphases exhibiting lagging of chromosomes indicated.

(H) Quantification of percentage of lagging chromatids that are the chromosome indicated from erroneous anaphases (144–307 lagging chromosomes analyzed per chromosome).

All experiments show mean ± SD of at least three independent experiments unless otherwise stated. ^∗∗p < 0.005 and ^∗∗∗∗p < 0.00005 (chi-square test; see Supplemental Experimental Procedures).