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. 2018 Jun 13;23(11):3366–3380. doi: 10.1016/j.celrep.2018.05.047

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© 2018 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Reversine Treatment Induces Different Biases

(A) RPE1 cells were treated with 250 nM reversine for 5 hr to induce lagging chromosomes before FISH with centromeric probes as indicated.

(B) Percentage anaphases with lagging chromosomes was quantified.

(C) Quantification of percentage of lagging chromatids (122–612 errors per chromosome analyzed) that are the chromosome indicated from erroneous anaphases. All experiments show mean ± SD of three experiments.

(D) Summary graph of conditions collated from Figures 2, 4, and 7. ^∗p < 0.05.