Fig. 2.

miR-29b-2-5p inhibits PDAC cell proliferation in vitro and in vivo experiments systems. a PDAC cell lines, SW1990 and Capan-2, were transfected with miR-29b-2-5p or NC. Cells were collected at 48, 24, 72, and 96 h after transfection using Trypan blue staining method. The results suggested miR-29b-2-5p significantly inhibited the proliferation of PDAC cells (mean ± SD, results of three independent experiments, *P < 0.05). b Observation under microscope of the cells transfected with miR-29b-2-5p or NC 72 h after transfection. The number of cells in miR-29b-2-5p group was significantly decreased compared with that in NC group. c miR-29b-2-5p agomir was intratumorally injected after the tumor was formed. After 2 weeks, the size of the subcutaneous tumor treated with miR-29b-2-5p agomir significantly decreased compared with NC-treated tumor. d Quantification of tumor volume development in NC- and miR-29b-2-5p-bearing nude mice. e Subcutaneous tumors derived from SW1990 cells in the NC- or miR-29b-2-5p agomir-treated group were weighed after tumors were harvested in histogram, *P < 0.05, **P < 0.001