Fig. 5.

MiR-29b-2-5p can promote cell p53 by suppressing Cbl-b, and Cbl-b can ubiquitination-dependent proteasomal degradation of p53. a SW1990 and Capan-2 cells were transfected with miR-29b-2-5p 48 h. Cell lysates were collected for Western blot analysis by p53 and p-p53; n = 3. b SW1990 cells were transfected with siCbl-b or OE Cbl-b 48 h. Cell lysates were collected for Western blot analysis by p53 and p-p53; n = 3. c The interaction of p53 with Cbl-b was analyzed by coimmunoprecipitation. d The OE Cbl-b cells were treated with PS341 for indicated times. p53 was immunoprecipitated and ubiquitin was analyzed by western blot. OE Cbl-b, Cbl-b plasmid transfected; NC, no expression plasmid controls; n = 3. e SW1990 cells were treated with 50 nmol/L miR-29b-2-5p or NC miRNA for 48 h. Protein localization in the cells was assessed using immunofluorescent staining. Cbl-b (green) and p53 (red), DAPI(blue), 40,6-diamidino-2-phenylindole