Fig. 5.

mKCTD9 interference inhibited hepatic NK cells activation and function. a Frequency of NK cells expressing KCTD9 as well as CD69 positive NK cells in liver were measured by FACS. b The Cytotoxicity of isolated hepatic NK cells from MHV-3 infected mice which were treated with mKCTD9 shRNA expressing plasmids or negative control. Cytotoxicity of NK cells calculated as following: (Experimental ALT – Spontaneous release ALT) / (Maximum ALT – Spontaneous release ALT) * 100%. c Frequency of IFN-γ and TNF-α expressing hepatic NK cells were measured by Flow cytometry. *p < 0.05, **p < 0.01, Means ± SEM of 3 to 5 independent experiments were represented