Table 1.

Primers used in mKCTD9 cloning, shRNA and quantitative PCR

Gene	Primer
Mouse KCTD9 (for cloning)	anti-sense 5’ ATAAGAATGCGGCCGCGCACCTCTGGGAACAAGC 3’
	sense 5’ CCCAAGCTTGTGCGTGCGTGAGGAGTGT 3’
mKCTD9-shRNA1	anti-sense 5’ AAGCTTAAAAATTGACCGAAGTCCTGAGTATCTCTTGAATACTCAGGACTTCGGTCAAAAACAAGGCTTTTC 3’
mKCTD9-shRNA2	anti-sense 5‘AAGCTTAAAAAGCCAGATGACAGGGATTAATCTCTTGAATTAATCCCTGTCATCTGGCAAACAAGGCTTTTC 3’
shRNA-Negative Control	anti-sense 5’ AAGCTTAAAAAGACGCAACGGAAGTGTTAATCTCTTGAATTAACACTTCCGTTGCGTCAAACAAGGCTTTTC 3’
mKCTD9-shRNA	sense 5’ GTAGGATCCATCCGACGCCGCCATCTC 3’
mKCTD9 (for qPCR)	anti-sense 5’ ACGATACTTCACAACAACAAGG 3’
	sense 5’ CCTGGCTTCTTCTAGCACAC 3’
GAPDH (for qPCR)	anti-sense 5’ CGGATTTGGTCGTATTGGG 3’
	sense 5’ CTCGCTCCTGGAAGATGG 3’