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. 2018 Jun 26;18:15. doi: 10.1186/s12861-018-0174-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Representative digital images of histogram profile shows DAB brown staining, color pixel intensity for Claudin-11 in the testes of adult mice, exposed to experimental diabetes mellitus and hypothyroidism. (A) From top to bottom rows; panels shows the digital image masks stained with hematoxylin, DAB and threshold respectively. (B) Claudin-11 is expressed in a limited quantity at tight junction in between Sertoli cells of all treated groups that is why analyzed through score calculation. Data are representing mean ± SEM (n = 6) and different labels indicated significant differences among groups at P < 0.05