Figure 1.
The RVD Sequences of the plTALEsTA and Their Target Sequences
(A) Scheme representing the different molecular parts of the pCR3.1-plTALEVP64 (the CMV promoter, N-ter, repeat domain, C-ter, NLS, and VP64) constructs. Primers P7p, TALE-F, TALE-R, and BGH were used for sequencing. (B) List of plTALEVP64, plTALEp300, plTALEST10X, and plTALEST24X names with their RVD sequences, which permit binding to the targeted nucleotides. (C) Schematic localization regions 1, 2, 3, 6, 7, 8, 10, 11, F2, F3, and F4 in the promoter and in intron 1 of the FXN gene targeted by the plTALEs.