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. 2018 May 21;16(1):1023–1030. doi: 10.3892/ol.2018.8755

Figure 4.

Figure 4.

The effect of STLC treatment on the expression of Eg5, cell apoptosis and cell cycle progression in NB. SK, SY5Y and BE2 cells were treated with 0, 1, 5, 10 and 20 mmol STLC for 72 h. (A) Flow cytometric analysis of the cell apoptosis. The percentage of apoptotic cells was dose-dependent in the NB cells. (B) The effect of STLC on the progression of the cell cycle in NB cells. The amplitude of curves corresponds to the cell number, the peak on the left (green) represents cells in the G1 phase of the cell cycle, while the peak on the right (blue) represents cells in the G2/M phase. Compared with cells treated with1 mmol STLC, the percentage of G2/M phase was significantly increased in higher concentrations. (C) Western blotting revealed that STLC treatment of SY5Y cells for 48 and 72 h resulted in a decrease of Eg5 expression. STLC, S-trityl-L-cysteine; NB, neuroblastoma; PI, propidium iodine.