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. 2018 May 18;16(1):835–842. doi: 10.3892/ol.2018.8736

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Effects of nonsense and CLCN3 antisense oligonucleotide transfection on CLCN3 mRNA and protein expression in U251 cells. (A) Densitometric analysis showing a significant decrease in CLCN3 mRNA expression induced by CLCN3 antisense transfection, whereas the transfection of a nonsense oligonucleotide did not significantly alter CLCN3 mRNA expression. (B) Representative RT-qPCR results are shown. β-actin was used as a loading control. The top panel shows the RT-qPCR and western blotting data for CLCN3 and β-actin from U251 cell lysates. The bottom panels show the integrated density values of CLCN3 relative to β-actin (n=3; P<0.05 vs. control). CLCN3, chloride voltage-gated channel 3.