Figure 2.

CLCN3 antisense reduces the viability of cisplatin-treated U251 cells. (A) Cell viability was measured by the MTT assay. Data are shown as means with standard deviations (SDs, bars) calculated from five separate experiments. (B) U251 cells treated with nonsense, CLCN3 antisense, Cisplatin or Cisplatin + CLCN3 antisense. Apoptotic nuclei (green) were identified by TUNEL staining (magnification, ×100). Data for the quantitative assessment of apoptosis are expressed as the mean apoptotic index ± SD. (C) Apoptotic cells stained positive for Annexin V-FITC and negative for PI (Q4 quadrant). Advanced apoptotic cells were stained positive for both Annnexin V-FITC and PI (Q2 quadrant). At advanced stages of apoptosis, cells were no longer viable. Data are presented as means ± SD from three independent experiments run in triplicate. (n=3; *P<0.05 vs. control group, ^#P<0.05 vs. cisplatin group). CLCN3, chloride voltage-gated channel 3; FITC, fluorescein isothiocyanate; PI, propidium iodide.