Figure 4.

Effects of cisplatin treatment and CLCN3 antisense transfection on matrix metalloproteinase expression, invasion and motility in U251 cells. Cells were transfected and treated with or without cisplatin (15 µmol/l) for 24 h. (A) MMP2 and MMP9 mRNA expression were detected by RT-qPCR. (B) Treated cells were detached and added to the upper compartment of an invasion chamber. The cells that invaded through the Matrigel were determined by light microscopy after 24 h (bar=100 µm). CLCN3, chloride voltage-gated channel 3; MMP, matrix metalloprotease.