Table II.
Status of different apoptosis relative genes in U251 cells.
| Groups | Control | CLCN3 antisense | Cisplatin | Cisplatin+CLCN3 antisense |
|---|---|---|---|---|
| p53/β-actin | 1.0±2.4 | 18.5±3.7a | 102.3±15.4a | 121.7±10.9a,b |
| Bcl-2/Bax | 1.0±0.12 | 1.02±0.24 | 0.36±0.14a | 0.24±0.08a,b |
| Pro-caspase 3/cleaved-caspase 3 | 1.0±0.47 | 1.10±0.32 | 4.49±0.91a | 6.68±0.84a,b |
Relative levels of different apoptosis relative genes calculated from intensities of bands are shown in Fig. 3A. Data show the means with standard deviations (SDs, bars) calculated from five separate experiments.
a
P<0.05 vs. control
b
P<0.05 vs. cisplatin by unpaired t-test. CLCN3, chloride voltage-gated channel 3; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated protein X.