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. 2018 May 22;16(1):949–955. doi: 10.3892/ol.2018.8770

Figure 6.

Figure 6.

miR-130a modulated apoptosis by downregulating DEPDC1 expression in HepG2 cells. HepG2 cells were transfected with miR-130a expressing plasmids or NC plasmids. (A) Cells were counted at 48 and 72 h, and the results indicated that miR-130a expression resulted in a significant decrease in the cell number, compared with miR-NC. (B) Apoptosis was analyzed by flow cytometry, and the results demonstrated that miR-130a expression enhanced apoptosis, compared with miR-NC. (C) miR-130a expression in cells transfected with miR-130a expressing plasmids or NC plasmids was examined by RT-qPCR, and the results demonstrated that miR-130a expression increased ~100-fold in cells transfected with miR-130a expressing plasmids, compared with miR-NC. (D) The DEPDC1 protein levels were significantly reduced 72 h post-transfection as demonstrated by western blot analysis data, compared with miR-NC. (E) The DEPDC1 mRNA levels were significantly reduced 72 h post-transfection as demonstrated by RT-qPCR data, compared with miR-NC. (F) A20 expression levels were increased significantly 72 h post-transfection, compared with miR-NC. These results indicated that miR-130a has the ability to regulate HepG2 apoptosis by targeting DEPDC1. The data are presented as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; NC, control; miR-130a, microRNA 130a; DEPDC1, DEP domain containing 1.