Figure 2.

Contribution of hypoxia-induced HIF-1α to ROS accumulation and glycolysis. (A) The knockdown efficiency of siRNA-1, siRNA-2 or siRNA-1/2 to HIF-1α was detected by western blot analysis. (B) ROS accumulation was detected following HIF-1α knockdown. Following HIF-1α knockdown, (C) ATP production, (D) glucose uptake and (E) lactate accumulation were detected following exposure to hypoxia after 12 h. *P<0.05 vs. normoxia. ROS, reactive oxygen species; siRNA, small interfering RNA; HIF-1α, hypoxia inducible facor-1α; NC, negative control.