Figure 3.

Effect of miR-142 on apoptosis-associated signaling molecules and tumor suppressors, Rb and PTEN. (A) The protein expression of cytosol Cyto c and caspase-3 cleaved. (B) The expression of PTEN and Rb and the phosphorylation status of p-Rb in MG-63 cells post 48 h transfection with different concentrations of miR-142. Left panel: Representative images; Right panel: Quantification of the gray scale of the bands in different groups using ImageJ software. (C) The gene expression of PTEN measured by reverse transcription-quantitative polymerase chain reaction. (D) The potential binding pattern of mir-142 to the rb gene. *P<0.05, **P<0.01, compared with Con and NC groups. miR, microRNA; NC, negative control; p-, phosphorylated-, PTEN, phosphatase and tensin homolog; Rb, Retinoblastoma-associated protein; Cyto c, cytochrome c; Con, blank control.