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. 2018 May 16;16(1):769–774. doi: 10.3892/ol.2018.8728

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Inhibitory effect of IL-33 on the growth of HCT-116 colon cancer cells. (A) The clonogenic survival assay of HCT-116 cells in the presence and absence of IL-33. The number of colonies was counted and expressed as a percentage of total colonies in the controls. (B) Immunocytochemistry for PCNA of HCT-116 cells in the presence and absence of IL-33. PCNA staining intensity was analyzed and results are expressed as the average integrated staining intensity relative to that in control cells. Original magnification, ×400. (C) Cell proliferation evaluated with a proliferation kit in the presence and absence of IL-33. Results are expressed as the mean OD ± standard error of the mean in each group. ^ÙP<0.05, compared with the control. OD, optical density; IL-33, interleukin 33; PCNA, proliferating cell nuclear antigen.