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. 2018 May 18;16(1):809–814. doi: 10.3892/ol.2018.8739

Figure 1.

Figure 1.

FGFRL1-deficient cells. (A) The sequence of the target site in the FGFRL1 gene. Following transfection of plasmid encoding Cas9 and RNAs, colonies were isolated and the sequences of the FGFRL1 gene were examined. No mutation was detected in the clone 1. A single nucleotide (thymidine) insertion was detected at the first ATG within the FGFRL1 gene in the clone 15. Deletion of 11 nucleotides, including the first ATG within the FGFRL1 gene, was observed in the clone 21. (B) Western blot analysis of KYSE520 cells. Whole cell extracts prepared from each cell line were analysed using SDS-PAGE. (C) Expression of FGFRs in wild-type and FGFRL1-deficient KYSE520 cells. Whole cell extracts prepared from each cell lines were analysed by SDS-PAGE. (D) Proliferation of cells. At the indicated times, cells were harvested with trypsin and counted. Data represent the mean of three experiments. Error bars represent the standard error of the mean. FGFRL1, fibroblast growth factor receptor-like 1; FGFR, fibroblast growth factor receptor; Nt, nucleotide residue.