Table 1.
Bone histomorphometric parameters of trabecular and cortical bones.
LA enhances both trabecular and cortical bone microarchitecture. Histomorphometric parameters of trabecular and cortical bones of LV5, femur and tibia. Bone volume/tissue volume ratio (BV/TV); Tb. Th., trabecular thickness; Tb. No., trabecular number; Conn. Den., Connectivity density; Tb. Sp., trabecular separation; Tb. Pf., trabecular pattern factor; Tt. Ar., total cross-sectional area; T. Pm., total cross-sectional perimeter; Ct. Ar., cortical bone area; B. Pm., bone perimeter; Ct. Th., average cortical thickness; MMI., polar moment of inertia. The results were evaluated by using ANOVA with subsequent comparisons by Student t-test for paired or nonpaired data as appropriate. Analysis was performed using Sigma plot software (Systat Software, Inc., Germany). Inner values are reported as mean ± SEM (n = 10) and similar results were obtained in three independent experiments. Statistical significance was defined as p ≤ 0.05 (*p ≤ 0.05, **p ≤ 0.001, ***p ≤ 0.0001) with respect to ovx mice group.
| Bone parameters | Sham | Ovx | Ovx + LA |
|---|---|---|---|
| LV5 | |||
| BV/TV (%) | 23.5 ± 0.04 | 16.53 ± 1.61 | 21.83 ± 0.04** |
| Tb. Th (mm) | 1.65 ± 0.08 | 0.64 ± 0.16 | 1.42 ± 0.07** |
| Tb. No (mm−1) | 4.67 ± 0.52 | 1.78 ± 0.04 | 4.15 ± 0.20*** |
| Conn. Den (mm−3) | 6.83 ± 1.34 | 2.78 ± 0.97 | 6.11 ± 1.22** |
| Tb. Sp. (mm) | 1.32 ± 0.08 | 2.54 ± 0.24 | 1.65 ± 0.04** |
| Cl. Po (%) | 8.15 ± 0.07 | 10.87 ± 0.24 | 8.21 ± 0.16** |
| Femur trabecular | |||
| BV/TV (%) | 30.71 ± 0.32 | 13.21 ± 3.21 | 25.41 ± 1.51** |
| Tb. Th (mm) | 1.73 ± 0.14 | 0.74 ± 0.16 | 1.45 ± 0.08** |
| Tb. No (mm−1) | 5.21 ± 0.11 | 2.33 ± 0.43 | 4.76 ± 0.08** |
| Conn. Den (mm−3) | 9.64 ± 0.68 | 4.51 ± 1.04 | 7.95 ± 0.06** |
| Tb. Sp. (mm) | 1.60 ± 0.51 | 3.84 ± 0.06 | 1.86 ± 0.08** |
| Cl. Po (%) | 3.02 ± 0.09 | 5.45 ± 0.21 | 3.32 ± 0.13** |
| Tibia trabecular | |||
| BV/TV (%) | 26.67 ± 0.35 | 20.11 ± 0.35 | 25.31 ± 0.24** |
| Tb. Th (mm) | 1.88 ± 0.02 | 1.10 ± 0.02 | 1.76 ± 0.03* |
| Tb. No (mm−1) | 4.60 ± 0.17 | 3.01 ± 0.48 | 4.25 ± 0.15** |
| Conn. Den (mm−3) | 4.45 ± 0.77 | 2.58 ± 0.76 | 4.26 ± 0.08** |
| Tb. Sp. (mm) | 1.87 ± 0.23 | 3.37 ± 0.07 | 2.10 ± 0.12** |
| Cl. Po (%) | 2.76 ± 0.04 | 4.96 ± 0.61 | 3.12 ± 0.25** |
| Femur cortical | |||
| Tt. Ar (mm2) | 2.76 ± 0.02 | 1.18 ± 0.10 | 2.55 ± 0.11** |
| T. Pm (mm) | 7.65 ± 1.21 | 4.22 ± 0.17 | 6.43 ± 0.07** |
| Ct. Ar (mm2) | 8.98 ± 0.21 | 3.51 ± 0.48 | 7.05 ± 0.11** |
| B. Pm (mm) | 1.12 ± 0.07 | 0.64 ± 0.10 | 1.10 ± 0.09** |
| Ct. Th (mm) | 1.22 ± 0.01 | 0.54 ± 0.06 | 1.09 ± 0.59** |
| MMI (mm4) | 2.42 ± 0.12 | 1.21 ± 0.25 | 2.24 ± 0.17** |
| Tibia cortical | |||
| Tt. Ar (mm2) | 4.88 ± 0.06 | 3.12 ± 0.91 | 4.80 ± 0.0.98** |
| T. Pm (mm) | 7.19 ± 0.12 | 5.12 ± 0.97 | 6.98 ± 0.57* |
| Ct. Ar (mm2) | 4.93 ± 0.32 | 3.18 ± 0.57 | 4.76 ± 0.32* |
| B. Pm (mm) | 8.69 ± 0.77 | 6.31 ± 0.14 | 8.23 ± 0.24* |
| Ct. Th (mm) | 7.78 ± 0.11 | 5.03 ± 0.89 | 7.43 ± 0.29*** |
| MMI (mm4) | 4.53 ± 0.67 | 3.12 ± 0.35 | 4.23 ± 0.52* |