Figure 4.

CAFs were disrupted by nab-paclitaxel in IH-CCA. (A) In order to study the effects of the treatments on CAFs, tissues from rats treated with the indicated agents were stained with α-SMA (red) and quantitated for the presence of activated and total fibroblasts. (B) The confocal microscopic analysis of α-SMA (red) and 4′6-diamidino-2-phenylindole (blue) immunofluorescence staining of total and activated CAFs in tumors treated with paclitaxel and nab-paclitaxel. CAFs, cancer-associated fibroblasts; IH-CCA, intrahepatic cholangiocarcinoma; α-SMA, α-smooth muscle actin.