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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Curr Protoc Chem Biol. 2018 Jun 7;10(2):e39. doi: 10.1002/cpch.39

Figure 2. Generation of affinity reagents through phage display.

Figure 2

A phage library is incubated with immobilized target protein. Non-binding phage are washed away, the remaining phage are eluted, amplified, and subjected to further rounds of selection. Then individual clones are amplified and analyzed for binding.