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. Author manuscript; available in PMC: 2019 Jun 7.
Published in final edited form as: Curr Protoc Chem Biol. 2018 Jun 7;10(2):e39. doi: 10.1002/cpch.39

Figure 3. Subcloning FN3 monobody coding regions into the pET14b-SUMO vector by SLiCE.

Figure 3

The FN3 coding sequence is amplified with a set of primers that add external sequences homologous to the regions bordering the cloning sites on the pET14b-Sumo vector. The insert is gel-purified and cloned into the destination vector via the SLiCE reaction.