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. Author manuscript; available in PMC: 2018 Jun 27.
Published in final edited form as: Adv Exp Med Biol. 2018;1029:101–107. doi: 10.1007/978-981-10-7545-2_9

Figure 1. Ciona Late Tailbud for multi-channel combinatorial FACS.

Figure 1

Ciona late tailbud expresses Mesp>tagRFP, MyoD905>GFP and Hand-r>tagBFP fluorescent reporters. Embryos are fixed at St. 25 and imaged using Leica TCS SP8 X confocal microscopy. a. Isolated single channel showing the Mesp>tagRFP labelled B7.5 lineage (TVC progeny and ATMs are in grey color) and the leaky expression in mesenchyme cells (faint grey signal); b. The overlapped channels of Mesp>tagRFP, MyoD905>GFP and Hand-r>tagBFP reporters. Mesp>tagRFP labeled B7.5 lineage cells are in red. MyoD905>GFP labelled mesenchyme cells are in green. Hand-r>tagBFP reporter labels only a portion of TVC progeny due to the mosaicism. The tagRFP-tagBFP+ cells, which appear in magenta (red overlaid with blue), will be sorted for further analysis.